DS LOGO

B.S05.0392 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

Jump to Record:



diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for B.S05.0392       Order Seed    View all Ds Insertions

Alt
Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 1: 644,432 GRMZM2G032104_T01
[View Locus Details]
0.2 kb from 3` end gi|133822407 (B.S05.0392_Lc18_JSR01_dir) MQ3 5d
1 1: 644,432 GRMZM2G032104_T01
[View Locus Details]
0.2 kb from 3` end gi|133822405 (B.S05.0392_JSR01) MQ2 5a

Placement Details

More on Insertion Site...


This seedstock is available from the Ac/Ds Project

To order seed, use the online order form . You will need a purchase order from your institution to complete the order. When ordering seed, use the 9-11 digit barcode as the unique identifier ( B.S05.0392). Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order.

Before You Order...


Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 133822405 (B.S05.0392_JSR01) Blast@ZmGDB

Show FASTA Sequence for gi|133822405 (689 bp)

>gi|133822405|gb|EI413781|B.S05.0392
GCGCTGGGTGGAAGACACGCGGCATGGGAGGCTTCCACGCGGAGCAGAAACATGGGCACGTACCCGCCCACTCGTCGGTGAGGCGTCCTCGCGTGTCTCC
CTCCCAATATCCATTCACTCCAGTCCTTATCCTATCTATCTATATATATATTATGCAAGAACCTACTAGCTGCTCCACTCATCTATTTCTTTTGAATCAG
CTAGCTAGCTAGCCAGAGCTTGTATGGCCACTGCTCATCATGCGGATGACCATCGTGCAGGGAGAAGAAGCGAGGCCGTCGGAGAGAACTACATGAGGGG
CCTCTACGGCGACGATGACTACAACGCCACCCACTACGGTCACCAGCAGCAGCAGCGGCCGCCGCCCGCGCCCATGGCGGTGGCCAAGGCGTTAGCCACA
GCCACGGCAGCCTTCTCCATGTTGTTGTTGTCGGGGCTGGCGGTGACGGGCACGGTGCTGGCGCTCATCGTGGCGACGCCATTGATGGTGATCTTCAGCC
CCGTGCTGGTGCCGGCGGCCATCACGGTGGCGCTGCTCACGGTAGGCATCGTTTCGTCGGGAGGGTTCGGCGTGGCTGCGGTGGCTGTGCTGGCGTGGGT
GTACCGCTACCTTCAGACCACGACCTCGTCGTCGGGTCAGCAGCCGCACATCGTCAAGGACTGGGCGCAGCAGCACCGTCTGGAGCAGA

5' Distal fDs: 133822407 (B.S05.0392_Lc18_JSR01_dir) Blast@ZmGDB

Show FASTA Sequence for gi|133822407 (557 bp)

>gi|133822407|gb|EI413782|B.S05.0392
AATAAAAAAAAAAAGCGTAGTACCCCTCCGTTTCTTTTTATTTGTTGCTGTTTAGTTCATATTTGTCACTGTTTCGTTAAAAATGAACTAGCGGGATAAC
GAGAACGGGAGGTAATATAGCTTTTCATTTAATGTAATGTAACGTAATCTTATGTTTGCAGCAGTAATAATGTGTATTATATATTTGCAGCAATAAAAGG
GCGAGCTCGATCGACCCCTCTACGAATAAATACTTTAAAAAACTTCTGATGCCGCTCCAAATCCAAATGCAGGCAGGCTTCAAGTACCAAATTGCTTACA
CAATAACACGTTACGATATTAGCACAAATAAATGAAGTAATATAGTCTGACTTGTACGTCTTGGTGGTGGGAAAGGAAGGTGGGCTAGCGTTCATTACTG
TCGGCCCAGCCCAGCCCAGGTCTTTCTCTCCTTCCTGTGAGTGCTGCATGTTGATTTCTACTCTCCCTCCCAGGTAGTAGTAGTAGTCTTCGTCGTCAAT
GGTCAATGGAGCAAAAACAAGCAACAAGCTAACGCTGATTGCGTTGTTGACGCGTGT



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 2.5 kb.
Southern Notes:

View Image: Southern Blot showing B.S05.0392
(Click image for larger version)

Southern Image

Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PvuII.
Predicted to produce a 1.5 kb band using primers LC24 LC18.
IPCR Notes:


Sequencing

IPCR product was processed by gel purified.
Sequencing reaction was performed using primers JSR01 and LC18.
Sequencing Notes: Two Reads

Loading Help Page...Thanks for your patience!

Loading Video...Thanks for your patience!

Loading Image...Thanks for your patience!