DS LOGO

I.S07.4236 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

Jump to Record:



Ds location and closest gene       Order Seed    View all Ds Insertions

Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr1:5,407,833 GRMZM2G176558_T01 -0.2 kb from 5` end gi|226938633 (I.S07.4236_JSR03) 5`Adj
1 Chr1:5,407,824 GRMZM2G176558_T01 -0.2 kb from 5` end gi|226938634 (I.S07.4236_JSR05) 3`Adj
(click for details)


Redundancy Group: RS.0138

(click for details)

How to request seedstock containing this insertion (click for details)


Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 226938633 (I.S07.4236_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|226938633 (101 bp)

>gi|226938633|gb|FI849955|I.S07.4236
GGGAGCGGGACAGCTCCCTCCCTCACTGCTGGAGGCTGGAGCGCATGCCGCCGCCTGCCTGTGCGGCTGTACCCGGTAGGCGGCTCGGCTGGCACCTGCA
G

3' Adjacent fDs: 226938634 (I.S07.4236_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938634 (1077 bp)

>gi|226938634|gb|FI849956|I.S07.4236
CCGCTCCCGCCCCTTCTACTTCTACCCCTCCCCCCCGCGGGCCGAGAGGGAGTCCGCCCGCCGGCGCCGCGCGACTCACCGATGCCCAGCCATGAGGCCT
GCTGCGGCACCATGTTCTGGGTGTACCTCATGTCCTGCGTCGGCCTCGTCGTCTTCGCCGGCCTCATGTCGGGCCTCACCCTCGGCCTCATGTCCCTCAG
CCTCGTCGACCTCGAGGTGCTCGCCAAGGCCGGCACGCCCAAGGACAAGCTCAACGCCGGTACTCCTCCTCCTCGTCTCTCCCGCGGCGGCGGCCGCATA
TTGCCGCACCAGCTGCTGCTTATGGTTTACCGACTAACTAACCTGTGTCGTGCGTTGTCCTCGCCCCCGCAGCCAGGATCCTCCCCGTAATGAAGAACCA
GCACCTGCTCCTCTGCACGCTCCTCATCGGAAACTCGCTCGCCATGGAGGCGCTGCCCATATTCCTCGACACGCTCGTGCCGCCCTACGTCGCCATCCTC
ATCTCCGTCACGCTCATCCTCGCGTTCGGGGAGGTGGGGCTGTCGTACCTCTACTGATCACTTCGATCGATCCAATGGAAATCAGTTTCCCCCCTTCCTG
ACGAATGAATTGATCCACCAGCTGATTCGGTTCCTCCTTCTGCTGTGCTACCTCCGCAGATCATGCCGCAAGCCATCTGCACGCGCTACGGGCTCAGCGT
GGGGGCCAAGGCGGCGCCCGTCGTCCGCCTACTGCTCATCCTCTTCTTCCCCGTCGCCTACCCGATCAGTAAGGTACGTTACGTGCGTGCTGTACCGGCG
GCTGATTTGTTCGTGTTTCAAACAGCTAGTGAGGGATTGGATTGGAGCCTTGTGCTTGCTGGATTGCCCGACTGAAACTGGGATGGGACAGCACAGGAGT
GGGCGGCCGCGAGTAGGATACAGATCGATCGCTACCTTGTCCATTATTTTAATTTGGCAACGGAAAGGGATTTTCGTTCCATGCTCGTCTAGTCGTCCAA
GTCACACAGGGCCAACACTGGCTCTTCTTCATCTAATAAATAATAAATAGATTGGATTGCGCATGCTGGCTCTGCAG



Genetics

Line was produced using a1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 3.4 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PstI.
Predicted to produce a 2.4 kb band using primers LC24 LC18.
IPCR Notes:


Sequencing

IPCR product was processed by ethanol precipitation.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes:

Loading Help Page...Thanks for your patience!

Loading Video...Thanks for your patience!

Loading Image...Thanks for your patience!