DS LOGO

I.S07.4140 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr1:12,299,471 GRMZM2G453139_T01 1.4 kb from 5` end gi|226938773 (I.S07.4140_JSR03) 5`Adj
1 Chr1:12,299,449 GRMZM2G453139_T01 1.3 kb from 5` end gi|226938906 (I.S07.4140_JSR05) 3`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 226938773 (I.S07.4140_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|226938773 (416 bp)

>gi|226938773|gb|FI849742|I.S07.4140
CCCACATGATCGCCACATGCACTTGTCCCCGTGAATTACTTCGCTTTCACTCTTTACCTCTGTCTGTATCTAGATATTACTATGTATTTTTTTTTAAAAA
AAAGAAAAGATGCATAGCTTTTACTACGGACTACCTCCAATATTATATATTTGACGCCGGATAGTGCATTTTTGAACTAAAAACGTAACAGAGGGAGTAT
CTATTAAAACCATGAGCACCCGCAAAATAACCACGCCACATCAGGCACACACGATGGCCTGTCCTAAACACATGGACGCCACAGTTCACTCGCTGCAACA
AACTGTCCAGCAGTGAGAGATGAACGAAGGCTTGACGGAACGAAAGGGACACACCGATGAGGGTTCACAAGGTCATCGGCCTCAATTATTCTGCCAATGA
CCCCCCCCCCCCCCCC

3' Adjacent fDs: 226938906 (I.S07.4140_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938906 (723 bp)

>gi|226938906|gb|FI849875|I.S07.4140
CATGCTGGGAGGATCGGAGCGCAATCAGTCAGTCCATGGGGGGGCTGGTTCCTGATGGAGGACGTGCAACAAACGCTCGCACACACAACACAAGACAAGC
ATAATGGAGCCTGCCTGCTTGCCGATCCGGGTCTCCAGACAGTTTCATGGCGCCAAGAGACTACGCCCAGGAGTGGAGAGGGAGAGCCATGTACATTTTT
GTGCTGGGCTGCCACATGAGGAAGAGACAAGGGGTGGAGAAGAGGATGCCATGCCACTAGCACTAGGTCCGCATTGGTCCGTGCGTCTCATTGTACTTGG
TCATGGTACAACCGCCTGTCGACCCCCAGGACAGGAAGCCATGCATGGTTGTACACACAGCACAGCTGGTGCTCCCGTTTCCCCCCCGCGCGCGTCCATG
CATGGGGGCCAAATACCCCAGCTGTACAAGCGTACCACCAACCTTTGCAGCTCATGAATGAGTAGCTAGCCCAGTACGTACGCAGACTTGAAACTGCTAG
GCCAGCAGCCAAGTGGCATATGTATGTTTATTTGTGATGTGTGCATGGTTAGGTCACAACGTACGGTGGCCTGCACACTTGGCACGGACGGTAGGGTCCG
GTCCCTCCTGTTGCCTGCCACACTACACCAGTGACTGGCGAGTAAGCAAATGAGCCACTTGGGGTTTGGGGTTGAGTCGAGTCGGAGTCGGACTCTGTGC
GTGTTGCTGTTGCACCAGCTGGT



Genetics

Line was produced using a1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 4.7 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PstI.
Predicted to produce a 3.2 kb band using primers LC24 LC18.
IPCR Notes:


Sequencing

IPCR product was processed by ethanol precipitation.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes:

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