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I.S07.3883A (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr1:2,453,540 GRMZM2G028870_T01 -0.9 kb from 5` end gi|226938790 (I.S07.3883A_JSR03) 5`Adj
1 Chr1:2,453,537 GRMZM2G028870_T01 -0.9 kb from 5` end gi|226938569 (I.S07.3883A_JSR05) 3`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 226938790 (I.S07.3883A_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|226938790 (148 bp)

>gi|226938790|gb|FI849759|I.S07.3883A
AGAATCAGGACAGCTTAGGCAGAGTCATCCTGTCTCTACAGTGAGGTAACAGACACAGTATGATATCCTGATCTTGGATACTATTATTTTTGCCATTTGA
TTGCTGACAACATATTGTTCTTGCCTAGGGAAGAAGTTCGCTCTGCAG

3' Adjacent fDs: 226938569 (I.S07.3883A_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938569 (355 bp)

>gi|226938569|gb|FI849891|I.S07.3883A
CTGATTCTCTGCTTGTGACAGAGATCGGCCTCTCATCATTCATAGACCTTCCGCTACGCAAGGATCGACCTCGTAGAAAAGACTGGAAAAACAATAGCGC
TCAATTCCATGAAATGCTCTACAAGACAATAATCTCAGCTAATTCTCCACAATAAAAAGGCTTCTTTGCATCGATATCGTTCAGAAGCAAGAAGAAAATC
TTCGGTTTCGGAGACGATGGAAGGAGAAGAAGAAAATGAAAGCGTTGGACCAAGTAAAAGATTGACGTTTGACGGCCGGGGGTTTTGGCCTAGACCTGAA
TGCGGCCGCGGTGAGCGAAGGCGGAGACGGCGCGGTGCTCGGAGAGGCCCTGCAG



Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of 0 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a 0 kb band using primers .
IPCR Notes:


Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers and .
Sequencing Notes:

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