DS LOGO

I.S07.2937 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Ds location and closest gene       Order Seed    View all Ds Insertions

Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr1:29,891,355 GRMZM2G135877_T01 0.8 kb from 5` end gi|226938822 (I.S07.2937_JSR03) 5`Adj
1 Chr1:29,891,364 GRMZM2G135877_T01 0.7 kb from 5` end gi|226938602 (I.S07.2937_JSR05) 3`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 226938822 (I.S07.2937_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|226938822 (260 bp)

>gi|226938822|gb|FI849791|I.S07.2937
CCCACCCCACCCCTCAAGCGCGCGCGTATATATATATGGGCAGGCCACAGCCCCCCCAAGGCGACCCAAACACAAACCAACCAGCACGACTCCTGAAAGC
CTGATCGCACTCCACTGACCGCCTCACCTCCTCTGGAGGAGACCAAAAGGCCGAGCACACCAGCACCAACCCAGAACAGAGAGAGAGAGAGAGAGAGAGA
GATTACTAAAAGGCTCGCGAGCAGGTTAGTCAAAACGTACTTGTATCTCGCGCCGGGAGC

3' Adjacent fDs: 226938602 (I.S07.2937_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938602 (724 bp)

>gi|226938602|gb|FI849924|I.S07.2937
GGGGTGGGGGGACGGACGGAGACGGACGCCAGGAGCGGCAGCATGCGCCGCCCCGCCCGCCCACGCACCAGCACCAGGCGGCACGGACGTACGTGTCCGC
CGTTGGGGGGTCGAACTGGACTGGAGGAGGCGCGCCCGCGGGCCGGGAGGGAGGAAGGCAAGTTCGCGTGCGCCGGCACCGGGCCCGATCACCTCTCGCG
TCTAGTAGGCATCTGCATCTATGCGCGCGCCTGGTTGTCTGAAAAATTCCGCTATCCCTTTGTGCGAGAGGAATGGCATATTGGCACGTCCGATCTGTCG
GAACAAATGGGTTCCAGTCTCTGGCGCCGCGTGCGTGCGTGCGCGCGCACCCACGGCCACTGGCCCGCCCCCGCCGTGACAGCTCAGTGGTCGATGCAGA
TTAACTCGACCGCCGCCCGAGTGGTTCGCGGCCGAACTGCACCAACACTGCTGTGACTGTAGAGTAGGCCGGCCGGGGGGGTGATTCGGTCGCAGTTGGC
CGCCGGCGCCTCACCAGGAGGGCCACCAGCCCGTAGTACGTAGCACCGGGGCACGGCACCAGCATTCCCGTTCCTCACGGAACAGATGGGCCCTGCCGTG
TTTTACTTTACCTTCATCACCACCTTCAATTTCTTCTGGCCTTGGGCCTACGCGCATAGCTCAACTGTCGACGTTGGAACGATGCATGCATGTCGACGAG
TCATTGCGCGCAGGGGCGGGGGCA



Genetics

Line was produced using a1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 4.7 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PstI.
Predicted to produce a 3 kb band using primers LC24 LC18.
IPCR Notes:


Sequencing

IPCR product was processed by precipitation.
Sequencing reaction was performed using primers JSR05 and JSR03.
Sequencing Notes:

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