DS LOGO

B.S05.0392 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr1:644,003 GRMZM2G333069_T01 0.2 kb from 5` end gi|133822407 (B.S05.0392_Lc18_JSR01_dir) 5`Dist
1 Chr1:644,003 GRMZM2G333069_T01 0.2 kb from 5` end gi|133822405 (B.S05.0392_JSR01) 5`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 133822405 (B.S05.0392_JSR01) Blast@ZmGDB

Show FASTA Sequence for gi|133822405 (689 bp)

>gi|133822405|gb|EI413781|B.S05.0392
GCGCTGGGTGGAAGACACGCGGCATGGGAGGCTTCCACGCGGAGCAGAAACATGGGCACGTACCCGCCCACTCGTCGGTGAGGCGTCCTCGCGTGTCTCC
CTCCCAATATCCATTCACTCCAGTCCTTATCCTATCTATCTATATATATATTATGCAAGAACCTACTAGCTGCTCCACTCATCTATTTCTTTTGAATCAG
CTAGCTAGCTAGCCAGAGCTTGTATGGCCACTGCTCATCATGCGGATGACCATCGTGCAGGGAGAAGAAGCGAGGCCGTCGGAGAGAACTACATGAGGGG
CCTCTACGGCGACGATGACTACAACGCCACCCACTACGGTCACCAGCAGCAGCAGCGGCCGCCGCCCGCGCCCATGGCGGTGGCCAAGGCGTTAGCCACA
GCCACGGCAGCCTTCTCCATGTTGTTGTTGTCGGGGCTGGCGGTGACGGGCACGGTGCTGGCGCTCATCGTGGCGACGCCATTGATGGTGATCTTCAGCC
CCGTGCTGGTGCCGGCGGCCATCACGGTGGCGCTGCTCACGGTAGGCATCGTTTCGTCGGGAGGGTTCGGCGTGGCTGCGGTGGCTGTGCTGGCGTGGGT
GTACCGCTACCTTCAGACCACGACCTCGTCGTCGGGTCAGCAGCCGCACATCGTCAAGGACTGGGCGCAGCAGCACCGTCTGGAGCAGA

5' Distal fDs: 133822407 (B.S05.0392_Lc18_JSR01_dir) Blast@ZmGDB

Show FASTA Sequence for gi|133822407 (557 bp)

>gi|133822407|gb|EI413782|B.S05.0392
AATAAAAAAAAAAAGCGTAGTACCCCTCCGTTTCTTTTTATTTGTTGCTGTTTAGTTCATATTTGTCACTGTTTCGTTAAAAATGAACTAGCGGGATAAC
GAGAACGGGAGGTAATATAGCTTTTCATTTAATGTAATGTAACGTAATCTTATGTTTGCAGCAGTAATAATGTGTATTATATATTTGCAGCAATAAAAGG
GCGAGCTCGATCGACCCCTCTACGAATAAATACTTTAAAAAACTTCTGATGCCGCTCCAAATCCAAATGCAGGCAGGCTTCAAGTACCAAATTGCTTACA
CAATAACACGTTACGATATTAGCACAAATAAATGAAGTAATATAGTCTGACTTGTACGTCTTGGTGGTGGGAAAGGAAGGTGGGCTAGCGTTCATTACTG
TCGGCCCAGCCCAGCCCAGGTCTTTCTCTCCTTCCTGTGAGTGCTGCATGTTGATTTCTACTCTCCCTCCCAGGTAGTAGTAGTAGTCTTCGTCGTCAAT
GGTCAATGGAGCAAAAACAAGCAACAAGCTAACGCTGATTGCGTTGTTGACGCGTGT



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 2.5 kb.
Southern Notes:

View Image: Southern Blot showing B.S05.0392
(Click image for larger version)

Southern Image

Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PvuII.
Predicted to produce a 1.5 kb band using primers LC24 LC18.
IPCR Notes:


Sequencing

IPCR product was processed by gel purified.
Sequencing reaction was performed using primers JSR01 and LC18.
Sequencing Notes: Two Reads

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